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Last updated: 2017/3/26
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PCK1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005105-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PCK1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 69.2
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PCK1 antibody (H00005105-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005105-T02
    PCK1 transfected lysate.
    Western Blot
    QC Testing of H00005105-T02
    Lane 1: PCK1 transfected lysate ( 69.20 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5105
  • Gene Name:
  • PCK1
  • Gene Alias:
  • MGC22652,PEPCK-C,PEPCK1,PEPCKC
  • Gene Description:
  • phosphoenolpyruvate carboxykinase 1 (soluble)
  • Gene Summary:
  • This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000031374,PEP carboxykinase,cytosolic phosphoenolpyruvate carboxykinase 1,phosphoenolpyruvate carboxylase,phosphopyruvate carboxylase
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