PCK1 (Human) IP-WB Antibody Pair
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of PCK1 transfected lysate using mouse monoclonal anti-PCK1 and Protein A Magnetic Bead (U0007), and immunoblotted with rabbit polyclonal anti-PCK1.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: mouse monoclonal anti-PCK1 (300 ug)
2. Antibody pair for WB: rabbit polyclonal anti-PCK1 (50 ul)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — PCK1
Entrez GeneID
5105Gene Name
PCK1
Gene Alias
MGC22652, PEPCK-C, PEPCK1, PEPCKC
Gene Description
phosphoenolpyruvate carboxykinase 1 (soluble)
Omim ID
261680Gene Ontology
HyperlinkGene Summary
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000031374|PEP carboxykinase|cytosolic phosphoenolpyruvate carboxykinase 1|phosphoenolpyruvate carboxylase|phosphopyruvate carboxylase
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