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Last updated: 2016/12/4

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PCK1 rabbit monoclonal antibody

  • Catalog # : H00005105-K
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against a human PCK1 peptide using ARM Technology.
  • Immunogen:
  • A synthetic peptide of human PCK1 is used for rabbit immunization.
    Customer or Abnova will decide on the preferred peptide sequence.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Purification:
  • Protein A
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody reactive against human PCK1 peptide by ELISA and mammalian transfected lysate by Western Blot.
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Note:
  • 1. Customer may provide cell or tissue lysate for antibody screening.
    2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.
  • Library Construction:
  • Non-fusion antibody library from rabbit spleen (ARM Technology).
  • Expression:
  • Overexpression vector and transfection into 293H cell line.
  • Deliverable:
  • Up to three rabbit IgG clones of 1 mg each will be delivered to customer.
  • Applications
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5105
  • GeneBank Accession#:
  • PCK1
  • Gene Name:
  • PCK1
  • Gene Alias:
  • MGC22652,PEPCK-C,PEPCK1,PEPCKC
  • Gene Description:
  • phosphoenolpyruvate carboxykinase 1 (soluble)
  • Gene Summary:
  • This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000031374,PEP carboxykinase,cytosolic phosphoenolpyruvate carboxykinase 1,phosphoenolpyruvate carboxylase,phosphopyruvate carboxylase
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