PCCB 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PCCB transfected lysate.
Western Blot
Lane 1: PCCB transfected lysate ( 59.4 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PCCB full-length
Host
Human
Theoretical MW (kDa)
59.4
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PCCB antibody (H00005096-B01) by Western Blots.
SDS-PAGE Gel
PCCB transfected lysate.
Western Blot
Lane 1: PCCB transfected lysate ( 59.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PCCB
Entrez GeneID
5096GeneBank Accession#
BC013768.1Protein Accession#
NP_000523.2Gene Name
PCCB
Gene Alias
DKFZp451E113
Gene Description
propionyl Coenzyme A carboxylase, beta polypeptide
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). [provided by RefSeq
Other Designations
PCCase subunit beta|propanoyl-CoA:carbon dioxide ligase subunit beta
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Interactome
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Pathway
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Disease
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