PCBP2 (Human) Recombinant Protein (P01)
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More Files
- More Functions
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Specification
Product Description
Human PCBP2 full-length ORF ( AAH01155, 1 a.a. - 362 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MDTGVIEGGLNVTLTIRLLMHGKEVGSIIGKKGESVKKMREESGARINISEGNCPERIITLAGPTNAIFKAFAMIIDKLEEDISSSMTNSTAASRPPVTLRLVVPASQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGIPQSIIECVKQICVVMLESPPKGVTIPYRPKPSSSPVIFAGGQDRYSTGSDSASFPHTTPSMCLNPDLEGPPLEAYTIQGQYAIPQPDLTKLHQLAMQQSHFPMTHGNTGFSGIESSSPEVKGYWAGLDASAQTTSHELTIPNDLIGCIIGRQGAKINEIRQMSGAQIKIANPVEGSTDRQVTITGSAASISLAQYLINVRLSSETGGMGSS
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
65.56
Interspecies Antigen Sequence
Mouse (100); Rat (99)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — PCBP2
Entrez GeneID
5094GeneBank Accession#
BC001155Protein Accession#
AAH01155Gene Name
PCBP2
Gene Alias
HNRPE2, MGC110998, hnRNP-E2
Gene Description
poly(rC) binding protein 2
Omim ID
601210Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
alpha-CP2|heterogenous nuclear ribonucleoprotein E2
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Interactome
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Publication Reference
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Binding of microRNA-122 to the hepatitis C virus 5' untranslated region modifies interactions with poly(C) binding protein 2 and the NS5B viral polymerase.
Seth Scott, You Li, Oya Bermek, Jack D Griffith, Stanley M Lemon, Kyung H Choi.
Nucleic Acids Research 2023 Dec; 51(22):12397.
Application:Pull-Down, N/A, Recombinant proteins.
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TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress.
Susu Guo, Yuxin Chen, Yueyue Yang, Xiao Zhang, Lifang Ma, Xiangfei Xue, Yongxia Qiao, Jiayi Wang.
Cell Death & Disease 2021 Jan; 12(1):42.
Application:PI, WB-Re, Recombinant proteins.
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CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer.
Liu Y, Zhang X, Lin J, Chen Y, Qiao Y, Guo S, Yang Y, Zhu G, Pan Q, Wang J, Sun F.
Cell Death & Disease 2019 Sep; 10(9):644.
Application:IF, IP-WB, WB-Tr, Human, Bel-7402, SMMC-7721 cells.
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A Combined ELONA-(RT)qPCR Approach for Characterizing DNA and RNA Aptamers Selected against PCBP-2.
Moreno M, Fernández-Algar M, Fernández-Chamorro J, Ramajo J, Martínez-Salas E, Briones C.
Molecules (Basel, Switzerland) 2019 Mar; 24(7):E1213.
Application:Func, RNA aptamers.
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Versatile Graphene-Based Platform for Robust Nanobiohybrid Interfaces.
Bueno R, Marciello M, Moreno M, Sánchez-Sánchez C, Martinez JI, Martinez L, Prats-Alfonso E, Guimerà-Brunet A, Garrido JA, Villa R, Mompean F, García-Hernandez M, Huttel Y, Del Puerto Morales M, Briones C, López MF, Ellis GJ, Vázquez L, Martín-Gago JA.
ACS Omega 2019 Feb; 4(2):3287.
Application:Aptamer-modified graphene surface, N/A, Recombinant protein.
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Poly(C)-binding protein 2 Interacts with sequences Required for Viral Replication in HCV 5' UTR and Directs HCV RNA Replication through Circularizing the Viral Genome.
Wang L, Jeng KS, Lai MM.
J Virol 2011 Jun; 85:7954.
Application:Func, Huh-7 RNA.
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Binding of microRNA-122 to the hepatitis C virus 5' untranslated region modifies interactions with poly(C) binding protein 2 and the NS5B viral polymerase.
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