PAFAH1B1 monoclonal antibody (M03), clone 5A5
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PAFAH1B1.
Immunogen
PAFAH1B1 (NP_000421, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDVNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5.
Lane 1: PAFAH1B1 transfected lysate(46.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PAFAH1B1 is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PAFAH1B1 on HeLa cell . [antibody concentration 15 ug/ml] -
Gene Info — PAFAH1B1
Entrez GeneID
5048GeneBank Accession#
NM_000430Protein Accession#
NP_000421Gene Name
PAFAH1B1
Gene Alias
LIS1, LIS2, MDCR, MDS, PAFAH
Gene Description
platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
Gene Ontology
HyperlinkGene Summary
This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq
Other Designations
Platelet-activating factor acetylhydrolase, isoform 1B, alpha subunit|lissencephaly 1 protein|platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit
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Interactome
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Pathway
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Disease
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Publication Reference
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The mechanism of acentrosomal spindle assembly in human oocytes.
Tianyu Wu, Jie Dong, Jing Fu, Yanping Kuang, Biaobang Chen, Hao Gu, Yuxi Luo, Ruihuan Gu, Meiling Zhang, Wen Li, Xi Dong, Xiaoxi Sun, Qing Sang, Lei Wang.
Science 2022 Nov; 378(6621):eabq7361.
Application:IF, Human, Human oocytes.
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A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.
So C, Seres KB, Steyer AM, Mönnich E, Clift D, Pejkovska A, Möbius W, Schuh M.
Science 2019 Jun; 364(6447):eaat9557.
Application:IF, Bovine, Mouse, Ovine, Pig, NIH/3T3 cells, Oocytes.
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The mechanism of acentrosomal spindle assembly in human oocytes.
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