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P4HB 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005034-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-P4HB full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 55.99
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-P4HB antibody (H00005034-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005034-T01
    P4HB transfected lysate.
    Western Blot
    QC Testing of H00005034-T01
    Lane 1: P4HB transfected lysate ( 55.99 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5034
  • Gene Name:
  • P4HB
  • Gene Alias:
  • DSI,ERBA2L,GIT,P4Hbeta,PDI,PDIA1,PHDB,PO4DB,PO4HB,PROHB
  • Gene Description:
  • prolyl 4-hydroxylase, beta polypeptide
  • Gene Summary:
  • This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq
  • Other Designations:
  • collagen prolyl 4-hydroxylase beta,glutathione-insulin transhydrogenase,procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide,prolyl 4-hydroxylase, beta subunit,protein disulfide isomerase family A, member 1,protein d
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