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Last updated: 2017/7/23

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P4HA1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005033-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-P4HA1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 58.85
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-P4HA1 antibody (H00005033-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005033-T01
    P4HA1 transfected lysate.
    Western Blot
    QC Testing of H00005033-T01
    Lane 1: P4HA1 transfected lysate ( 58.85 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5033
  • Gene Name:
  • P4HA1
  • Gene Alias:
  • P4HA
  • Gene Description:
  • prolyl 4-hydroxylase, alpha polypeptide I
  • Gene Summary:
  • This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
  • Other Designations:
  • C-P4Halpha(I),collagen prolyl 4-hydroxylase alpha(I),procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I,prolyl 4-hydroxylase, alpha I subunit
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