ORC4L 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ORC4L transfected lysate
Western Blot
Lane 1: ORC4L transfected lysate ( 50.4 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ORC4L full-length
Host
Human
Theoretical MW (kDa)
50.4
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ORC4L antibody (H00005000-B01) by Western Blots.
SDS-PAGE Gel
ORC4L transfected lysate
Western Blot
Lane 1: ORC4L transfected lysate ( 50.4 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ORC4L
Entrez GeneID
5000GeneBank Accession#
NM_002552Protein Accession#
NP_002543Gene Name
ORC4L
Gene Alias
ORC4, ORC4P
Gene Description
origin recognition complex, subunit 4-like (yeast)
Omim ID
603056Gene Ontology
HyperlinkGene Summary
The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown to form a core complex with ORC2L, -3L, and -5L. Three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
Other Designations
origin recognition complex subunit 4
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Interactome
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Pathway
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Disease
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