ODC1 monoclonal antibody (M01J), clone 2G5
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Specifications
Product Description
Mouse monoclonal antibody raised against a full-length recombinant ODC1.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
ODC1 (AAH25296, 1 a.a. ~ 461 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGAGFDCASKTEIQLVQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQTGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKYYSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGFQRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRHRAACASASINV
Host
Mouse
Reactivity
Human
Preparation Method
Cell Culture Production
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (76.45 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ODC1 monoclonal antibody (M01J), clone 2G5. Western Blot analysis of ODC1 expression in A-431.Western Blot (Transfected lysate)
Western Blot analysis of ODC1 expression in transfected 293T cell line by ODC1 monoclonal antibody (M01J), clone 2G5.
Lane 1: ODC1 transfected lysate (Predicted MW: 51.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of ODC1 transfected lysate using anti-ODC1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ODC1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ODC1 is 10 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to ODC1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — ODC1
Entrez GeneID
4953GeneBank Accession#
BC025296Protein Accession#
AAH25296Gene Name
ODC1
Gene Alias
ODC
Gene Description
ornithine decarboxylase 1
Gene Ontology
HyperlinkGene Summary
This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. [provided by RefSeq
Other Designations
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