ODC1 monoclonal antibody (M01), clone 2G5
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More Files
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Western Blot (Tissue lysate)
ODC1 monoclonal antibody (M01), clone 2G5. Western Blot analysis of ODC1 expression in human spleen.
Western Blot (Tissue lysate)
ODC1 monoclonal antibody (M01), clone 2G5. Western Blot analysis of ODC1 expression in rat brain.
Western Blot (Cell lysate)
ODC1 monoclonal antibody (M01), clone 2G5. Western Blot analysis of ODC1 expression in A-431.
Western Blot (Transfected lysate)
Western Blot analysis of ODC1 expression in transfected 293T cell line by ODC1 monoclonal antibody (M01), clone 2G5.
Lane 1: ODC1 transfected lysate(51.148 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of ODC1 transfected lysate using anti-ODC1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ODC1 MaxPab rabbit polyclonal antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to ODC1 on HeLa cell . [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ODC1.
Immunogen
ODC1 (AAH25296, 1 a.a. ~ 461 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGAGFDCASKTEIQLVQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQTGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKYYSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGFQRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRHRAACASASINV
Host
Mouse
Reactivity
Human, Rat
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
ODC1 monoclonal antibody (M01), clone 2G5. Western Blot analysis of ODC1 expression in human spleen.Western Blot (Tissue lysate)
ODC1 monoclonal antibody (M01), clone 2G5. Western Blot analysis of ODC1 expression in rat brain.Western Blot (Cell lysate)
ODC1 monoclonal antibody (M01), clone 2G5. Western Blot analysis of ODC1 expression in A-431.Western Blot (Transfected lysate)
Western Blot analysis of ODC1 expression in transfected 293T cell line by ODC1 monoclonal antibody (M01), clone 2G5.
Lane 1: ODC1 transfected lysate(51.148 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of ODC1 transfected lysate using anti-ODC1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ODC1 MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to ODC1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — ODC1
Entrez GeneID
4953GeneBank Accession#
BC025296Protein Accession#
AAH25296Gene Name
ODC1
Gene Alias
ODC
Gene Description
ornithine decarboxylase 1
Gene Ontology
HyperlinkGene Summary
This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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TWIST1 is a direct transcriptional target of MYCN and MYC in neuroblastoma.
Selmi A, de Saint-Jean M, Jallas AC, Garin E, Hogarty MD, Benard J, Puisieux A, Marabelle A, Valsesia-Wittmann S.
Cancer Letters 2015 Feb; 357(1):412.
Application：WB, Human, TET21 cells.
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TWIST1 is a direct transcriptional target of MYCN and MYC in neuroblastoma.
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