OCLN 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
OCLN transfected lysate.
Western Blot
Lane 1: OCLN transfected lysate ( 59.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-OCLN full-length
Host
Human
Theoretical MW (kDa)
59.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-OCLN antibody (H00004950-B01) by Western Blots.
SDS-PAGE Gel
OCLN transfected lysate.
Western Blot
Lane 1: OCLN transfected lysate ( 59.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — OCLN
Entrez GeneID
4950GeneBank Accession#
NM_002538.2Protein Accession#
NP_002529.1Gene Name
OCLN
Gene Alias
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Gene Description
occludin
Omim ID
602876Gene Ontology
HyperlinkGene Summary
This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq
Other Designations
tight junction protein occludin TM4 minus
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