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Last updated: 2016/10/16
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NT5E monoclonal antibody (M01), clone 4C4-2B5

  • Catalog # : H00004907-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full length recombinant NT5E.
  • Immunogen:
  • NT5E (AAH15940.1, 28 a.a. ~ 264 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00004907-M01
    Western Blot detection against Immunogen (51.81 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of NT5E expression in transfected 293T cell line by NT5E monoclonal antibody (M01), clone 4C4-2B5.

    Lane 1: NT5E transfected lysate(30.36 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Gene Information
  • Entrez GeneID:
  • 4907
  • Gene Name:
  • NT5E
  • Gene Alias:
  • CD73,E5NT,NT,NT5,NTE,eN,eNT
  • Gene Description:
  • 5'-nucleotidase, ecto (CD73)
  • Gene Summary:
  • Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM
  • Other Designations:
  • 5' nucleotidase (CD73),5' nucleotidase, ecto,OTTHUMP00000016808,OTTHUMP00000040565,Purine 5-Prime-Nucleotidase,ecto-5'-nucleotidase
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