NRF1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NRF1 transfected lysate.
Western Blot
Lane 1: NRF1 transfected lysate ( 55.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NRF1 full-length
Host
Human
Theoretical MW (kDa)
55.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NRF1 antibody (H00004899-D01P) by Western Blots.
SDS-PAGE Gel
NRF1 transfected lysate.
Western Blot
Lane 1: NRF1 transfected lysate ( 55.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NRF1
Entrez GeneID
4899GeneBank Accession#
BC016925.1Protein Accession#
AAH16925.1Gene Name
NRF1
Gene Alias
ALPHA-PAL
Gene Description
nuclear respiratory factor 1
Omim ID
600879Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq
Other Designations
OTTHUMP00000184912|alpha palindromic-binding protein
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Interactome
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