NPPB 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NPPB full-length
Host
Human
Theoretical MW (kDa)
14.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NPPB antibody (H00004879-D01P) by Western Blots.
SDS-PAGE Gel
NPPB transfected lysate.
Western Blot
Lane 1: NPPB transfected lysate ( 14.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NPPB
Entrez GeneID
4879GeneBank Accession#
NM_002521Protein Accession#
NP_002512.1Gene Name
NPPB
Gene Alias
BNP
Gene Description
natriuretic peptide precursor B
Omim ID
600295Gene Ontology
HyperlinkGene Summary
This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq
Other Designations
OTTHUMP00000002506|OTTHUMP00000044486|brain type natriuretic peptide|natriuretic protein
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