NOS3 monoclonal antibody (M04), clone 1D12
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NOS3.
Immunogen
NOS3 (AAH63294.1, 61 a.a. ~ 160 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAAT
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NOS3 is 3 ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between AKT1 and NOS3. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-NOS3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — NOS3
Entrez GeneID
4846GeneBank Accession#
BC063294Protein Accession#
AAH63294.1Gene Name
NOS3
Gene Alias
ECNOS, eNOS
Gene Description
nitric oxide synthase 3 (endothelial cell)
Gene Ontology
HyperlinkGene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
endothelial nitric oxidase synthase|endothelial nitric oxide synthase
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Interactome
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Disease
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