NNMT monoclonal antibody (M03), clone 2F2
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NNMT.
Immunogen
NNMT (AAH00234, 1 a.a. ~ 264 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85); Rat (87)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (54.78 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NNMT expression in transfected 293T cell line by NNMT monoclonal antibody (M03), clone 2F2.
Lane 1: NNMT transfected lysate(29.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of NNMT transfected lysate using anti-NNMT monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NNMT MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NNMT is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — NNMT
Entrez GeneID
4837GeneBank Accession#
BC000234Protein Accession#
AAH00234Gene Name
NNMT
Gene Alias
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Gene Description
nicotinamide N-methyltransferase
Omim ID
600008Gene Ontology
HyperlinkGene Summary
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq
Other Designations
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Interactome
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Disease
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