NMT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NMT1 transfected lysate.
Western Blot
Lane 1: NMT1 transfected lysate ( 56.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NMT1 full-length
Host
Human
Theoretical MW (kDa)
56.8
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NMT1 antibody (H00004836-B01) by Western Blots.
SDS-PAGE Gel
NMT1 transfected lysate.
Western Blot
Lane 1: NMT1 transfected lysate ( 56.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NMT1
Entrez GeneID
4836GeneBank Accession#
NM_021079Protein Accession#
NP_066565Gene Name
NMT1
Gene Alias
NMT
Gene Description
N-myristoyltransferase 1
Omim ID
160993Gene Ontology
HyperlinkGene Summary
Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM
Other Designations
alternative, short form NMT-S|long form, NMT-L|myristoyl-CoA:protein N-myristoyltransferase
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Interactome
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Disease
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