NME4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NME4 transfected lysate.
Western Blot
Lane 1: NME4 transfected lysate ( 20.68 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NME4 full-length
Host
Human
Theoretical MW (kDa)
20.68
Interspecies Antigen Sequence
Mouse (82); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NME4 antibody (H00004833-B01) by Western Blots.
SDS-PAGE Gel
NME4 transfected lysate.
Western Blot
Lane 1: NME4 transfected lysate ( 20.68 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NME4
Entrez GeneID
4833GeneBank Accession#
NM_005009.2Protein Accession#
NP_005000.1Gene Name
NME4
Gene Alias
NDPK-D, NM23H4, nm23-H4
Gene Description
non-metastatic cells 4, protein expressed in
Omim ID
601818Gene Ontology
HyperlinkGene Summary
The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM
Other Designations
NDP kinase D|nucleoside-diphosphate kinase 4
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