NME1 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of NME1 transfected lysate using mouse monoclonal anti-NME1 and Protein A Magnetic Bead (U0007), and immunoblotted with rabbit polyclonal anti-NME1.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94%); Rat (95%)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of NME1 transfected lysate using mouse monoclonal anti-NME1 and Protein A Magnetic Bead (U0007), and immunoblotted with rabbit polyclonal anti-NME1.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: mouse monoclonal anti-NME1 (300 ug)
2. Antibody pair for WB: rabbit polyclonal anti-NME1 (50 ul)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — NME1
Entrez GeneID
4830Gene Name
NME1
Gene Alias
AWD, GAAD, NB, NBS, NDPK-A, NDPKA, NM23, NM23-H1
Gene Description
non-metastatic cells 1, protein (NM23A) expressed in
Gene Ontology
HyperlinkGene Summary
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
Other Designations
NDP kinase A|OTTHUMP00000174772
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Interactome
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Pathway
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Disease
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