Western blot analysis of NME1 over-expressed 293 cell line, cotransfected with NME1 Validated Chimera RNAi ( Cat # H00004830-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NME1 monoclonal antibody (M01), clone 2H1 (Cat # H00004830-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq