NIT1 monoclonal antibody (M01), clone 1C3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.
Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NIT1.
Immunogen
NIT1 (NP_005591.1, 228 a.a. ~ 327 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
AFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.
Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — NIT1
Entrez GeneID
4817GeneBank Accession#
NM_005600Protein Accession#
NP_005591.1Gene Name
NIT1
Gene Alias
MGC57670
Gene Description
nitrilase 1
Omim ID
604618Gene Ontology
HyperlinkOther Designations
OTTHUMP00000029696
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