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Last updated: 2017/3/26
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NIT1 monoclonal antibody (M01), clone 1C3

  • Catalog # : H00004817-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant NIT1.
  • Immunogen:
  • NIT1 (NP_005591.1, 228 a.a. ~ 327 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • AFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00004817-M01
    Western Blot detection against Immunogen (36.74 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.

    Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NIT1 monoclonal antibody.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4817
  • Gene Name:
  • NIT1
  • Gene Alias:
  • MGC57670
  • Gene Description:
  • nitrilase 1
  • Other Designations:
  • OTTHUMP00000029696
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