NFYB (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human NFYB full-length ORF ( AAH05317, 1 a.a. - 207 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
48.51
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — NFYB
Entrez GeneID
4801GeneBank Accession#
BC005317Protein Accession#
AAH05317Gene Name
NFYB
Gene Alias
CBF-A, CBF-B, HAP3, NF-YB
Gene Description
nuclear transcription factor Y, beta
Omim ID
189904Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq
Other Designations
CCAAT-binding transcription factor subunit A|Transcription factor NF-Y, B subunit
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Interactome
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Pathway
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