NFYB monoclonal antibody (M01), clone 6H6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.
Lane 1: NFYB transfected lysate(22.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NFYB is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi ( Cat # H00004801-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 (Cat # H00004801-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (48.51 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NFYB.
Immunogen
NFYB (AAH05317, 1 a.a. ~ 207 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (48.51 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.
Lane 1: NFYB transfected lysate(22.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NFYB is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi ( Cat # H00004801-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 (Cat # H00004801-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — NFYB
Entrez GeneID
4801GeneBank Accession#
BC005317Protein Accession#
AAH05317Gene Name
NFYB
Gene Alias
CBF-A, CBF-B, HAP3, NF-YB
Gene Description
nuclear transcription factor Y, beta
Omim ID
189904Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq
Other Designations
CCAAT-binding transcription factor subunit A|Transcription factor NF-Y, B subunit
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Interactome
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Pathway
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