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Last updated: 2017/9/17

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NFYA rabbit monoclonal antibody

  • Catalog # : H00004800-K
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against a human NFYA peptide using ARM Technology.
  • Immunogen:
  • A synthetic peptide of human NFYA is used for rabbit immunization.
    Customer or Abnova will decide on the preferred peptide sequence.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Purification:
  • Protein A
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody reactive against human NFYA peptide by ELISA and mammalian transfected lysate by Western Blot.
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Note:
  • 1. Customer may provide cell or tissue lysate for antibody screening.
    2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.
  • Library Construction:
  • Non-fusion antibody library from rabbit spleen (ARM Technology).
  • Expression:
  • Overexpression vector and transfection into 293H cell line.
  • Deliverable:
  • Up to three rabbit IgG clones of 1 mg each will be delivered to customer.
  • Applications
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4800
  • GeneBank Accession#:
  • NFYA
  • Gene Name:
  • NFYA
  • Gene Alias:
  • CBF-A,CBF-B,FLJ11236,HAP2,NF-YA
  • Gene Description:
  • nuclear transcription factor Y, alpha
  • Gene Summary:
  • The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq
  • Other Designations:
  • CAAT-box DNA binding protein subunit A,CCAAT-binding transcription factor subunit B,HAP2 CCAAT-binding protein,OTTHUMP00000016365,Transcription factor NF-Y, A subunit
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