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Last updated: 2016/10/23
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NFKB1 monoclonal antibody (M01), clone 2E6

  • Catalog # : H00004790-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant NFKB1.
  • Immunogen:
  • NFKB1 (AAH51765, 860 a.a. ~ 969 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00004790-M01
    Western Blot detection against Immunogen (37.73 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M01), clone 2E6.

    Lane 1: NFKB1 transfected lysate(105.4 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged NFKB1 is approximately 10ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of NFKB1 over-expressed 293 cell line, cotransfected with NFKB1 Validated Chimera RNAi ( Cat # H00004790-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFKB1 monoclonal antibody (M01), clone 2E6 (Cat # H00004790-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • PDF DownloadProtocol Download
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Proximity Ligation Analysis of protein-protein interactions between IKBKB and NFKB1. HeLa cells were stained with anti-IKBKB rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4790
  • Gene Name:
  • NFKB1
  • Gene Alias:
  • DKFZp686C01211,EBP-1,KBF1,MGC54151,NF-kappa-B,NFKB-p105,NFKB-p50,p105,p50
  • Gene Description:
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
  • Gene Summary:
  • This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • DNA binding factor KBF1,NF-kappabeta,nuclear factor NF-kappa-B p50 subunit,nuclear factor kappa-B DNA binding subunit,nuclear factor kappa-B, subunit 1
  • Interactome
  • Interactome
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