NFIL3 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human NFIL3 full-length ORF ( AAH08197, 1 a.a. - 462 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MQLRKMQTVKKEQASLDASSNVDKMMVLNSALTEVSEDSTTGEELLLSEGSVGKNKSSACRRKREFIPDEKKDAMYWEKRRKNNEAAKRSREKRRLNDLVLENKLIALGEENATLKAELLSLKLKFGLISSTAYAQEIQKLSNSTAVYFQDYQTSKSNVSSFVDEHEPSMVSSSCISVIKHSPQSSLSDVSEVSSVEHTQESSVQGSCRSPENKFQIIKQEPMELESYTREPRDDRGSYTASIYQNYMGNSFSGYSHSPPLLQVNRSSSNSPRTSETDDGVVGKSSDGEDEQQVPKGPIHSPVELKHVHATVVKVPEVNSSALPHKLRIKAKAMQIKVEAFDNEFEATQKLSSPIDMTSKRHFELEKHSAPSMVHSSLTPFSVQVTNIQDWSLKSEHWHQKELSGKTQNSFKTGVVEMKDSGYKVSDPENLYLKQGIANLSAEVVSLKRLIATQPISASDSG
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
76.56
Interspecies Antigen Sequence
Mouse (85); Rat (84)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — NFIL3
Entrez GeneID
4783GeneBank Accession#
BC008197Protein Accession#
AAH08197Gene Name
NFIL3
Gene Alias
E4BP4, IL3BP1, NF-IL3A, NFIL3A
Gene Description
nuclear factor, interleukin 3 regulated
Omim ID
605327Gene Ontology
HyperlinkGene Summary
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).[supplied by OMIM
Other Designations
OTTHUMP00000021633|interleukin-3 promoter transcriptional activator
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Interactome
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Disease
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Publication Reference
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A novel E4BP4 element drives circadian expression of mPeriod2.
Ohno T, Onishi Y, Ishida N.
Nucleic Acids Research 2006 Dec; 35(2):648.
Application:Func, Mouse, NIH/3T3 cells.
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A novel E4BP4 element drives circadian expression of mPeriod2.
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