NFIC monoclonal antibody (M01), clone 1C12-2A2
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More Files
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Western Blot (Cell lysate)
NFIC monoclonal antibody (M01), clone 1C12-2A2 Western Blot analysis of NFIC expression in A-431 ( Cat # L015V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.
Lane 1: NFIC transfected lysate(47.6 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
RNAi Knockdown (Antibody validated)
Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi ( Cat # H00004782-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody (M01), clone 1C12-2A2 (Cat # H00004782-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (72.82 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NFIC.
Immunogen
NFIC (AAH12120, 1 a.a. ~ 428 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSCRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQHHRPVIAVHSGIARSPHPSSALHFPTTSILPQTASTYFPHTAIRYPPHLNPQDPLKDLVSLACDPASQQPGPSWYLG
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (72.82 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NFIC monoclonal antibody (M01), clone 1C12-2A2 Western Blot analysis of NFIC expression in A-431 ( Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.
Lane 1: NFIC transfected lysate(47.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi ( Cat # H00004782-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody (M01), clone 1C12-2A2 (Cat # H00004782-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — NFIC
Entrez GeneID
4782GeneBank Accession#
BC012120Protein Accession#
AAH12120Gene Name
NFIC
Gene Alias
CTF, CTF5, MGC20153, NF-I, NFI
Gene Description
nuclear factor I/C (CCAAT-binding transcription factor)
Omim ID
600729Gene Ontology
HyperlinkOther Designations
CCAAT-binding transcription factor|nuclear factor I/C
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Interactome
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Disease
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Publication Reference
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NFI-C2 temporal-spatial expression and cellular localization pattern during tooth formation.
Lamani E, Gluhak-Heinrich J, MacDougall M.
Development, Growth & Differentiation 2015 Dec; 57(9):625.
Application：ICC, IHC-P, WB-Ce, Human, Mouse, Dental cells, Tooth.
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NFI-C2 temporal-spatial expression and cellular localization pattern during tooth formation.
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