NFE2L2 monoclonal antibody (M03), clone 1B8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NFE2L2.
Immunogen
NFE2L2 (AAH11558, 71 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
FAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCMQLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (73); Rat (72)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NFE2L2 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NFE2L2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — NFE2L2
Entrez GeneID
4780GeneBank Accession#
BC011558Protein Accession#
AAH11558Gene Name
NFE2L2
Gene Alias
NRF2
Gene Description
nuclear factor (erythroid-derived 2)-like 2
Omim ID
600492Gene Ontology
HyperlinkGene Summary
NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM
Other Designations
NF-E2-related factor 2|OTTHUMP00000164988
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Interactome
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Disease
- Adenoma
- Alzheimer Disease
- Asthma
- Atherosclerosis
- Breast cancer
- Breast Neoplasms
- Calcinosis
- Carcinoma
- Cataract
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Publication Reference
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Lithium promotes recovery after spinal cord injury.
Ying-Jie Zhao, Hao Qiao, Dong-Fan Liu, Jie Li, Jia-Xi Li, Su-E Chang, Teng Lu, Feng-Tao Li, Dong Wang, Hao-Peng Li, Xi-Jing He, Fang Wang.
Neural Regeneration Research 2022 Jun; 17(6):1324.
Application:IF, Rat, PC-12 cells, Rat spinal cord.
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Examining the endogenous antioxidant response through immunofluorescent analysis of Nrf2 in tissue.
Lindl KA, Jordan-Sciutto KL.
Methods in Molecular Biology 2008 Dec; 477:229.
Application:IF, Human, Hippocampus.
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Lithium promotes recovery after spinal cord injury.
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