NFIA monoclonal antibody (M02), clone 1E11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of NFIA expression in transfected 293T cell line by NFIA monoclonal antibody (M02), clone 1E11.
Lane 1: NFIA transfected lysate (Predicted MW: 54.6 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to NFIA on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (80.52 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NFIA.
Immunogen
NFIA (AAH22264, 1 a.a. ~ 498 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKSVEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPNGSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPDTKPPTTSTEGGAASPTSPILVPGIKVAASHHPPDRPPDPFSTL
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (80.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NFIA expression in transfected 293T cell line by NFIA monoclonal antibody (M02), clone 1E11.
Lane 1: NFIA transfected lysate (Predicted MW: 54.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NFIA on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — NFIA
Entrez GeneID
4774GeneBank Accession#
BC022264Protein Accession#
AAH22264Gene Name
NFIA
Gene Alias
DKFZp434L0422, KIAA1439, NFI-L
Gene Description
nuclear factor I/A
Omim ID
600727Gene Ontology
HyperlinkGene Summary
Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.[supplied by OMIM
Other Designations
OTTHUMP00000010292
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Interactome
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Disease
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Publication Reference
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The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation.
Rosa A, Ballarino M, Sorrentino A, Sthandier O, De Angelis FG, Marchioni M, Masella B, Guarini A, Fatica A, Peschle C, Bozzoni I.
PNAS 2007 Dec; 104(50):19849.
Application：WB, Human, NB4 cells.
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The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation.
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