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NEK3 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00004752-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-NEK3 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 55.77
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-NEK3 antibody (H00004752-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004752-T01
    NEK3 transfected lysate.
    Western Blot
    QC Testing of H00004752-T01
    Lane 1: NEK3 transfected lysate ( 55.77 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4752
  • Gene Name:
  • NEK3
  • Gene Alias:
  • HSPK36,MGC29949
  • Gene Description:
  • NIMA (never in mitosis gene a)-related kinase 3
  • Gene Summary:
  • This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene
  • Other Designations:
  • NIMA-related kinase 3,glycogen synthase A kinase,hydroxyalkyl-protein kinase,phosphorylase B kinase kinase,serine/threonine-protein kinase NEK3
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