NEK3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NEK3 transfected lysate.
Western Blot
Lane 1: NEK3 transfected lysate ( 55.77 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NEK3 full-length
Host
Human
Theoretical MW (kDa)
55.77
Interspecies Antigen Sequence
Mouse (74); Rat (69)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NEK3 antibody (H00004752-B01) by Western Blots.
SDS-PAGE Gel
NEK3 transfected lysate.
Western Blot
Lane 1: NEK3 transfected lysate ( 55.77 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NEK3
Entrez GeneID
4752GeneBank Accession#
NM_002498.1Protein Accession#
NP_002489.1Gene Name
NEK3
Gene Alias
HSPK36, MGC29949
Gene Description
NIMA (never in mitosis gene a)-related kinase 3
Omim ID
604044Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene
Other Designations
NIMA-related kinase 3|glycogen synthase A kinase|hydroxyalkyl-protein kinase|phosphorylase B kinase kinase|serine/threonine-protein kinase NEK3
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Interactome
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Disease
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