NEK3 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of NEK3 transfected lysate using rabbit polyclonal anti-NEK3 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-NEK3.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (74%); Rat (69%)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of NEK3 transfected lysate using rabbit polyclonal anti-NEK3 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-NEK3.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-NEK3 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-NEK3 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — NEK3
Entrez GeneID
4752Gene Name
NEK3
Gene Alias
HSPK36, MGC29949
Gene Description
NIMA (never in mitosis gene a)-related kinase 3
Omim ID
604044Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene
Other Designations
NIMA-related kinase 3|glycogen synthase A kinase|hydroxyalkyl-protein kinase|phosphorylase B kinase kinase|serine/threonine-protein kinase NEK3
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Interactome
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Disease
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