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Last updated: 2017/7/23

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NEK3 rabbit monoclonal antibody

  • Catalog # : H00004752-K
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against a human NEK3 peptide using ARM Technology.
  • Immunogen:
  • A synthetic peptide of human NEK3 is used for rabbit immunization.
    Customer or Abnova will decide on the preferred peptide sequence.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Purification:
  • Protein A
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody reactive against human NEK3 peptide by ELISA and mammalian transfected lysate by Western Blot.
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Note:
  • 1. Customer may provide cell or tissue lysate for antibody screening.
    2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.
  • Library Construction:
  • Non-fusion antibody library from rabbit spleen (ARM Technology).
  • Expression:
  • Overexpression vector and transfection into 293H cell line.
  • Deliverable:
  • Up to three rabbit IgG clones of 1 mg each will be delivered to customer.
  • Applications
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4752
  • GeneBank Accession#:
  • NEK3
  • Gene Name:
  • NEK3
  • Gene Alias:
  • HSPK36,MGC29949
  • Gene Description:
  • NIMA (never in mitosis gene a)-related kinase 3
  • Gene Summary:
  • This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene
  • Other Designations:
  • NIMA-related kinase 3,glycogen synthase A kinase,hydroxyalkyl-protein kinase,phosphorylase B kinase kinase,serine/threonine-protein kinase NEK3
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