NEK3 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Tissue lysate)
NEK3 MaxPab polyclonal antibody. Western Blot analysis of NEK3 expression in human colon.
Western Blot (Cell lysate)
NEK3 MaxPab polyclonal antibody. Western Blot analysis of NEK3 expression in A-431.
Western Blot (Transfected lysate)
Western Blot analysis of NEK3 expression in transfected 293T cell line (H00004752-T01) by NEK3 MaxPab polyclonal antibody.
Lane 1: NEK3 transfected lysate(55.66 KDa).
Lane 2: Non-transfected lysate.
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human NEK3 protein.
Immunogen
NEK3 (NP_002489.1, 1 a.a. ~ 506 a.a) full-length human protein.
Sequence
MDDYMVLRMIGEGSFGRALLVQHESSNQMFAMKEIRLPKSFSNTQNSRKEAVLLAKMKHPNIVAFKESFEAEGHLYIVMEYCDGGDLMQKIKQQKGKLFPEDMILNWFTQMCLGVNHIHKKRVLHRDIKSKNIFLTQNGKVKLGDFGSARLLSNPMAFACTYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCTLKHPFQANSWKNLILKVCQGCISPLPSHYSYELQFLVKQMFKRNPSHRPSATTLLSRGIVARLVQKCLPPEIIMEYGEEVLEEIKNSKHNTPRKKTNPSRIRIALGNEASTVQEEEQDRKGSHTDLESINENLVESALRRVNREEKGNKSVHLRKASSPNLHRRQWEKNVPNTALTALENASILTSSLTAEDDRGGSVIKYSKNTTRKQWLKETPDTLLNILKNADLSLAFQTYTIYRPGSEGFLKGPLSEETEASDSVDGGHDSVILDPERLEPGLDEEDTDFEEEDDNPDWVSELKKRAGWQGLCDR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (74); Rat (69)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
NEK3 MaxPab polyclonal antibody. Western Blot analysis of NEK3 expression in human colon.Western Blot (Cell lysate)
NEK3 MaxPab polyclonal antibody. Western Blot analysis of NEK3 expression in A-431.Western Blot (Transfected lysate)
Western Blot analysis of NEK3 expression in transfected 293T cell line (H00004752-T01) by NEK3 MaxPab polyclonal antibody.
Lane 1: NEK3 transfected lysate(55.66 KDa).
Lane 2: Non-transfected lysate.
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Gene Info — NEK3
Entrez GeneID
4752GeneBank Accession#
NM_002498.1Protein Accession#
NP_002489.1Gene Name
NEK3
Gene Alias
HSPK36, MGC29949
Gene Description
NIMA (never in mitosis gene a)-related kinase 3
Omim ID
604044Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene
Other Designations
NIMA-related kinase 3|glycogen synthase A kinase|hydroxyalkyl-protein kinase|phosphorylase B kinase kinase|serine/threonine-protein kinase NEK3
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Interactome
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Disease
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