NEK3 (Human) Matched Antibody Pair
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Specification
Product Description
This antibody pair set comes with a matched antibody pair to detect and quantify the protein level of human NEK3.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (74%); Rat (69%)
Quality Control Testing
Standard curve using NEK3 293T overexpression lysate (non-denatured) as an analyte.
Sandwich ELISA detection sensitivity ranging from approximately 729x to 3x dilution of the NEK3 293T overexpression lysate (non-denatured).
Supplied Product
Antibody pair set content:
1. Capture antibody: mouse monoclonal anti-NEK3 (100 ug)
2. Detection antibody: rabbit purified polyclonal anti-NEK3 (50 ug)
*Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
ELISA Pair (Transfected lysate)
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Gene Info — NEK3
Entrez GeneID
4752Gene Name
NEK3
Gene Alias
HSPK36, MGC29949
Gene Description
NIMA (never in mitosis gene a)-related kinase 3
Omim ID
604044Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene
Other Designations
NIMA-related kinase 3|glycogen synthase A kinase|hydroxyalkyl-protein kinase|phosphorylase B kinase kinase|serine/threonine-protein kinase NEK3
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Interactome
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Disease
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