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Last updated: 2016/11/27

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NEFL 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00004747-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-NEFL full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 59.84
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-NEFL antibody (H00004747-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004747-T01
    NEFL transfected lysate.
    Western Blot
    QC Testing of H00004747-T01
    Lane 1: NEFL transfected lysate ( 59.84 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4747
  • Protein Accession#:
  • -
  • Gene Name:
  • NEFL
  • Gene Alias:
  • CMT1F,CMT2E,FLJ53642,NF-L,NF68,NFL
  • Gene Description:
  • neurofilament, light polypeptide
  • Gene Summary:
  • Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq
  • Other Designations:
  • light molecular weight neurofilament protein,neurofilament protein, light chain,neurofilament subunit NF-L,neurofilament triplet L protein,neurofilament, light polypeptide 68kDa,neurofilament-light
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