Western blot analysis of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi ( Cat # H00004724-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody (M01), clone 1A1 (Cat # H00004724-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes. [provided by RefSeq
NADH dehydrogenase (ubiquinone) Fe-S protein 4,NADH dehydrogenase (ubiquinone) iron-sulfur protein 4,NADH-coenzyme Q reductase, 18-KD,NADH-ubiquinone oxidoreductase 18 kDa subunit,mitochondrial respiratory chain complex I (18-KD subunit)