NDUFS2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NDUFS2 transfected lysate.
Western Blot
Lane 1: NDUFS2 transfected lysate ( 51.04 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NDUFS2 full-length
Host
Human
Theoretical MW (kDa)
51.04
Interspecies Antigen Sequence
Mouse (92); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NDUFS2 antibody (H00004720-B01) by Western Blots.
SDS-PAGE Gel
NDUFS2 transfected lysate.
Western Blot
Lane 1: NDUFS2 transfected lysate ( 51.04 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NDUFS2
Entrez GeneID
4720GeneBank Accession#
NM_004550.3Protein Accession#
NP_004541.1Gene Name
NDUFS2
Gene Alias
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Gene Description
NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
Gene Ontology
HyperlinkGene Summary
Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM
Other Designations
NADH dehydrogenase (ubiquinone) Fe-S protein 2|NADH-ubiquinone oxidoreductase Fe-S protein 2|NADH-ubiquinone oxidoreductase NDUFS2 subunit|OTTHUMP00000032241
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Interactome
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Pathway
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Disease
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