NDUFS1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NDUFS1 full-length
Host
Human
Theoretical MW (kDa)
79.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NDUFS1 antibody (H00004719-B01) by Western Blots.
SDS-PAGE Gel
NDUFS1 transfected lysate.
Western Blot
Lane 1: NDUFS1 transfected lysate ( 79.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NDUFS1
Entrez GeneID
4719GeneBank Accession#
NM_005006.5Protein Accession#
-Gene Name
NDUFS1
Gene Alias
CI-75Kd, MGC26839, PRO1304
Gene Description
NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq
Other Designations
NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa|NADH-coenzyme Q reductase|complex I, mitochondrial respiratory chain, 75-kD subunit
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Interactome
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Pathway
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Disease
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