NDUFS1 purified MaxPab mouse polyclonal antibody (B01P)
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human NDUFS1 protein.
Immunogen
NDUFS1 (NP_004997.4, 1 a.a. ~ 727 a.a) full-length human protein.
Sequence
MLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQSFQGKDVAAIAGGLVDAEALVALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYTYDHLGDSPKILQDIASGSHPFSQVLKEAKKPMVVLGSSALQRNDGAAILAAVSSIAQKIRMTSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKVLFLLGADGGCITRQDLPKDCFIIYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDIEGANYFQQANELSKLVNQQLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC
Host
Mouse
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
NDUFS1 MaxPab polyclonal antibody. Western Blot analysis of NDUFS1 expression in human spleen.Western Blot (Transfected lysate)
Western Blot analysis of NDUFS1 expression in transfected 293T cell line (H00004719-T01) by NDUFS1 MaxPab polyclonal antibody.
Lane 1: NDUFS1 transfected lysate(79.97 KDa).
Lane 2: Non-transfected lysate.
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Gene Info — NDUFS1
Entrez GeneID
4719GeneBank Accession#
NM_005006.5Protein Accession#
NP_004997.4Gene Name
NDUFS1
Gene Alias
CI-75Kd, MGC26839, PRO1304
Gene Description
NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq
Other Designations
NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa|NADH-coenzyme Q reductase|complex I, mitochondrial respiratory chain, 75-kD subunit
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Interactome
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Pathway
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Disease
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Publication Reference
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Human Ind1, an iron-sulfur cluster assembly factor for respiratory complex I.
Sheftel AD, Stehling O, Pierik AJ, Netz DJ, Kerscher S, Elsasser HP, Wittig I, Balk J, Brandt U, Lill R.
Molecular and Cellular Biology 2009 Nov; 29(22):6059.
Application:WB-Tr, Human, HeLa cells.
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Human Ind1, an iron-sulfur cluster assembly factor for respiratory complex I.
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