NDUFA8 monoclonal antibody (M05), clone 2E10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of NDUFA8 expression in transfected 293T cell line by NDUFA8 monoclonal antibody (M05), clone 2E10.
Lane 1: NDUFA8 transfected lysate (Predicted MW: 20.1 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to NDUFA8 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NDUFA8 is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (33.66 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NDUFA8.
Immunogen
NDUFA8 (NP_055037.1, 1 a.a. ~ 72 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPGIVELPTLEELKVDEVKISSAVLKAAAHHYGAQCDKPNKEFMLCRWEEKDPRRCLEEGKLVNKCALDFFR
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (33.66 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NDUFA8 expression in transfected 293T cell line by NDUFA8 monoclonal antibody (M05), clone 2E10.
Lane 1: NDUFA8 transfected lysate (Predicted MW: 20.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to NDUFA8 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NDUFA8 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — NDUFA8
Entrez GeneID
4702GeneBank Accession#
NM_014222Protein Accession#
NP_055037.1Gene Name
NDUFA8
Gene Alias
CI-19KD, CI-PGIV, MGC793, PGIV
Gene Description
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa
Omim ID
603359Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the complex I 19 kDA subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq
Other Designations
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (19kD, PGIV)|NADH-ubiquinone oxidoreductase 19 kDa subunit|NADH:ubiquinone oxidoreductase PGIV subunit|OTTHUMP00000022034
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Interactome
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Pathway
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Disease
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