NDUFA5 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NDUFA5 full-length
Host
Human
Theoretical MW (kDa)
13.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NDUFA5 antibody (H00004698-D01P) by Western Blots.
SDS-PAGE Gel
NDUFA5 transfected lysate.
Western Blot
Lane 1: NDUFA5 transfected lysate ( 13.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NDUFA5
Entrez GeneID
4698GeneBank Accession#
NM_005000.2Protein Accession#
NP_004991.1Gene Name
NDUFA5
Gene Alias
B13, CI-13KD-B, DKFZp781K1356, FLJ12147, NUFM, UQOR13
Gene Description
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
Omim ID
601677Gene Ontology
HyperlinkGene Summary
The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq
Other Designations
Complex I-13KD-B|NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5|NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (13kD, B13)|type I dehydrogenase|ubiquinone reductase
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Interactome
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