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Last updated: 2017/12/10

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NDUFA5 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00004698-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-NDUFA5 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 12.87
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-NDUFA5 antibody (H00004698-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004698-T01
    NDUFA5 transfected lysate.
    Western Blot
    QC Testing of H00004698-T01
    Lane 1: NDUFA5 transfected lysate ( 12.87 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4698
  • Gene Name:
  • NDUFA5
  • Gene Alias:
  • B13,CI-13KD-B,DKFZp781K1356,FLJ12147,NUFM,UQOR13
  • Gene Description:
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
  • Gene Summary:
  • The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq
  • Other Designations:
  • Complex I-13KD-B,NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5,NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (13kD, B13),type I dehydrogenase,ubiquinone reductase
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