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Last updated: 2017/4/23

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NDUFA5 rabbit monoclonal antibody

  • Catalog # : H00004698-K
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against a human NDUFA5 peptide using ARM Technology.
  • Immunogen:
  • A synthetic peptide of human NDUFA5 is used for rabbit immunization.
    Customer or Abnova will decide on the preferred peptide sequence.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Purification:
  • Protein A
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody reactive against human NDUFA5 peptide by ELISA and mammalian transfected lysate by Western Blot.
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Note:
  • 1. Customer may provide cell or tissue lysate for antibody screening.
    2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.
  • Library Construction:
  • Non-fusion antibody library from rabbit spleen (ARM Technology).
  • Expression:
  • Overexpression vector and transfection into 293H cell line.
  • Deliverable:
  • Up to three rabbit IgG clones of 1 mg each will be delivered to customer.
  • Applications
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4698
  • Gene Name:
  • NDUFA5
  • Gene Alias:
  • B13,CI-13KD-B,DKFZp781K1356,FLJ12147,NUFM,UQOR13
  • Gene Description:
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
  • Gene Summary:
  • The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq
  • Other Designations:
  • Complex I-13KD-B,NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5,NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (13kD, B13),type I dehydrogenase,ubiquinone reductase
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