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NDUFA1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00004694-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-NDUFA1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 8.1
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-NDUFA1 antibody (H00004694-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004694-T02
    NDUFA1 transfected lysate.
    Western Blot
    QC Testing of H00004694-T02
    Lane 1: NDUFA1 transfected lysate ( 8.10 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4694
  • Gene Name:
  • NDUFA1
  • Gene Alias:
  • CI-MWFE,MWFE,ZNF183
  • Gene Description:
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
  • Gene Summary:
  • The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq
  • Other Designations:
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1,NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1 (7.5kD, MWFE),NADH oxidoreductase subunit MWFE,NADH:ubiquinone oxidoreductase (complex 1),OTTHUMP00000023930,type I dehydrogenase
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