NCK1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: NCK1 transfected lysate ( 42.9 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
NCK1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NCK1 full-length
Host
Human
Theoretical MW (kDa)
41.58
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NCK1 antibody (H00004690-B01) by Western Blots.
Western Blot
Lane 1: NCK1 transfected lysate ( 42.9 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
NCK1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NCK1
Entrez GeneID
4690GeneBank Accession#
NM_006153Protein Accession#
NP_006144Gene Name
NCK1
Gene Alias
MGC12668, NCK, NCKalpha
Gene Description
NCK adaptor protein 1
Omim ID
600508Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq
Other Designations
NCK tyrosine kinase|SH2/SH3 adaptor protein NCK-alpha|melanoma NCK protein|non-catalytic region of tyrosine kinase
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Interactome
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Pathway
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