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NCF4 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00004689-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-NCF4 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 37.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-NCF4 antibody (H00004689-B01) by Western Blots.
    Western Blot
    QC Testing of H00004689-T01
    Lane 1: NCF4 transfected lysate ( 39 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00004689-T01
    NCF4 transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4689
  • Gene Name:
  • NCF4
  • Gene Alias:
  • MGC3810,NCF,P40PHOX,SH3PXD4
  • Gene Description:
  • neutrophil cytosolic factor 4, 40kDa
  • Gene Summary:
  • The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000028736,OTTHUMP00000043756,neutrophil NADPH oxidase factor 4,neutrophil cytosol factor 4,neutrophil cytosolic factor 4
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