NCF2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NCF2 full-length
Host
Human
Theoretical MW (kDa)
57.97
Interspecies Antigen Sequence
Mouse (85); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NCF2 antibody (H00004688-B01) by Western Blots.
Western Blot
Lane 1: NCF2 transfected lysate ( 57.86 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
NCF2 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NCF2
Entrez GeneID
4688GeneBank Accession#
BC001606Protein Accession#
AAH01606Gene Name
NCF2
Gene Alias
FLJ93058, NCF-2, NOXA2, P67-PHOX, P67PHOX
Gene Description
neutrophil cytosolic factor 2
Gene Ontology
HyperlinkGene Summary
This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in two transcript variants that encode the same protein. [provided by RefSeq
Other Designations
67 kDa neutrophil oxidase factor|NADPH oxidase activator 2|OTTHUMP00000033269|OTTHUMP00000033270|chronic granulomatous disease, autosomal 2|neutrophil cytosol factor 2|neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
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Interactome
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Pathway
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Disease
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