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PPP1R12B 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00004660-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PPP1R12B full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 42.57
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PPP1R12B antibody (H00004660-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00004660-T01
    PPP1R12B transfected lysate
    Western Blot
    QC Testing of H00004660-T01
    Lane 1: PPP1R12B transfected lysate ( 42.57 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 4660
  • Gene Name:
  • PPP1R12B
  • Gene Alias:
  • MGC131980,MGC87886,MYPT2
  • Gene Description:
  • protein phosphatase 1, regulatory (inhibitor) subunit 12B
  • Gene Summary:
  • Myosin light chain phosphatase (MLCP) consists of three subunits- catalytic subunit, large subunit/myosin binding subunit (MBS) and small subunit (sm-M20). This gene is a multi-functional gene which encodes both MBS and sm-M20. MLCP regulates myosins and the dephosphorylation is enhanced by the presence of MBS. The sm-M20 is suggested to play a regulatory role in muscle contraction by binding to MBS. MBS is also encoded by another gene, myosin light chain phosphatase target subunit 1. sm-M20 shows higher binding affinity to this gene product than to myosin light chain phosphatase target subunit 2-MBS even though the two MBS proteins are highly similar. Although both MBSs increase the activity of MLCP, myosin light chain phosphatase target subunit 1-MBS is a more efficient activator. There are four alternatively spliced transcript variants described; two alter the MBS coding region and two alter the sm-M20 coding region of this gene. [provided by RefSeq
  • Other Designations:
  • myosin phosphatase regulatory subunit,myosin phosphatase, target subunit 2
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