MYOC monoclonal antibody (M01), clone 4F8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MYOC expression in transfected 293T cell line by MYOC monoclonal antibody (M01), clone 4F8.
Lane 1: MYOC transfected lysate(57 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MYOC on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
Immunoprecipitation
Immunoprecipitation of MYOC transfected lysate using anti-MYOC monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYOC MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MYOC is approximately 0.3ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of MYOC over-expressed 293 cell line, cotransfected with MYOC Validated Chimera RNAi ( Cat # H00004653-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYOC monoclonal antibody (M01), clone 4F8 (Cat # H00004653-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MYOC.
Immunogen
MYOC (AAH29261, 36 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QLRKANDQSGRCQYTFSVASPNESSCPEQSQAMSVIHNLQRDSSTQRLDLEATKARLSSLESLLHQLTLDQAARPQETQEGLQRELGTLRRERDQLETQT
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MYOC expression in transfected 293T cell line by MYOC monoclonal antibody (M01), clone 4F8.
Lane 1: MYOC transfected lysate(57 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MYOC on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of MYOC transfected lysate using anti-MYOC monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYOC MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MYOC is approximately 0.3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MYOC over-expressed 293 cell line, cotransfected with MYOC Validated Chimera RNAi ( Cat # H00004653-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYOC monoclonal antibody (M01), clone 4F8 (Cat # H00004653-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MYOC
Entrez GeneID
4653GeneBank Accession#
BC029261Protein Accession#
AAH29261Gene Name
MYOC
Gene Alias
GLC1A, GPOA, JOAG, JOAG1, TIGR, myocilin
Gene Description
myocilin, trabecular meshwork inducible glucocorticoid response
Gene Ontology
HyperlinkGene Summary
MYOC encodes the protein myocilin, which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma. [provided by RefSeq
Other Designations
OTTHUMP00000032681|mutated trabecular meshwork-induced glucocorticoid response protein|myocilin
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Interactome
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Disease
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Publication Reference
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Magnetic Nano-Platform Enhanced iPSC-Derived Trabecular Meshwork Delivery and Tracking Efficiency.
Xiangji Wang, Qilong Cao, Shen Wu, Mohammad Reza Bahrani Fard, Ningli Wang, Jie Cao, Wei Zhu.
International Journal of Nanomedicine 2022 Mar; 17:1285.
Application：WB, Human, Mouse, Human TM cells, Mouse iPSC.
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Glucocorticoid Receptor Transactivation Is Required for Glucocorticoid-Induced Ocular Hypertension and Glaucoma.
Patel GC, Millar JC, Clark AF.
Investigative Ophthalmology & Visual Science 2019 May; 60(6):1967.
Application：WB, Mouse, MTM cells.
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Glucocorticoid receptor GRβ regulates glucocorticoid-induced ocular hypertension in mice.
Patel GC, Liu Y, Millar JC, Clark AF.
Scientific Reports 2018 Jan; 8(1):862.
Application：WB, Mouse, Mouse eyes.
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Magnetic Nano-Platform Enhanced iPSC-Derived Trabecular Meshwork Delivery and Tracking Efficiency.
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